Research Article

Phenethyl Isothiocyanate (PEITC) Inhibits the Growth of Human Oral Squamous Carcinoma HSC-3 Cells through G0/G1 Phase Arrest and Mitochondria-Mediated Apoptotic Cell Death

Figure 5

PEITC altered the levels of 𝐺 0 / 𝐺 1 phase and apoptotic relative proteins in HSC-3 cells. Cells were exposed to PEITC (2.5 μM) and then incubated for 0, 6, 12 24, 48, and 72 h. Then cells were harvested for total protein determination then the protein levels of p15, cdc25A, CDK6, and cyclin D (a), p53, p27, p21, CDK2, and cyclin E (b), Fas, FasL, and caspase 8 (c), Bcl-2, Bax, Bid, and xIAP (d), cytochrome 𝑐 , caspase 9, and 3 (e), AIF, Endo G, and NF-κB p65 (f), GRP78 and caspase 4 (g) were determined protein levels by Western blotting, and then protein levels were quantified by NIH ImageJ software. Data are presented from three independent experiments with similar results.
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