Explore the Molecular Mechanism of Apoptosis Induced by Tanshinone IIA on Activated Rat Hepatic Stellate Cells

Table 1

Differentially expressed proteins of hepatic stellate cells follow tanshinone IIA treated.

Spot number	Protein name	Accession number	Mw (kDa)	pI	Coverage (score)	Matched peptide	MS/MS fragment	Ratio^a	P value^b	Biological function
1	TCTP	P63029	19.56	4.88	40 (97%)	9	^6DLISHDELFSDIYK^19 (71)	−2.63±0.02	0.01	Involved in calcium binding and microtubule stabilization
2	PSA5	P34064	26.55	4.79	36 (66%)	7	^11GVNTFSPEGR^20 (25)	−2.18±0.06	0.02	The proteasome has an ATP-dependent proteolytic activity
3	14-3-3E	P62260	29.33	4.63	47 (57%)	7	^131YLAEFATGNDRK^142 (31)	−3.12±0.05	0.03	Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways
4	PRDX2	P35704	19.90	5.29	45 (102%)	11	^140QITVNDLPVGR^150 (26)	2.58±0.04	0.05	Involved in redox regulation of the cell
5	PEBP1	P31044	20.90	5.48	71 (94%)	12	^81FREWHHFLVVNMK^93 (29)	2.83±0.06	0.03	Binds ATP, opioids, and phosphatidylethanolamine
6	PHB	P67779	29.86	5.57	92 (279%)	26	^94ILFRPVASQLPR^105 (80); ^106IYTSIGEDYDER^117 (44); ^134FDAGELITQR^143 (50)	3.25±0.08	0.01	Prohibitin inhibits DNA synthesis
7	HSPB1	P42930	22.94	6.12	62 (118%)	13	^29LFDQAFGVPR^38 (52)	3.92±0.10	0.03	Involved in stress resistance and actin organization
8	PRDX6	O35244	24.86	5.64	53 (106%)	12	^25FHDFLGDSWGILFSHPR^41 (35); ^42DFTPVCTTELGR^53 (42); ^133VVFIFGPDKK^142 (35)	2.42±0.04	0.04	Involved in redox regulation of the cell
9	PRDX3	Q9Z0V6	28.56	7.14	73 (37%)	8	^172DYGVLLESAGIALR^185 (26); ^198HLSVNDLPVGR^208 (30)	2.28±0.04	0.04	Involved in redox regulation of the cell
10	GDIR1	Q5XI73	23.45	5.12	70 (135%)	15	^59VAVSADPNVPNVIVTR^74 (34); ^100QSFVLKEGVEYR^111 (41)	2.56±0.03	0.04	Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them
11	ATP5H	P31399	16.08	8.92	59 (90%)	7	^33SWNETFHTR^41 (28)	3.38±0.08	0.02	Mitochondrial membrane ATP synthase
12	ALDH2	P11884	56.97	6.63	88 (27%)	15	^162TIPIDGDFFSYTR^174 (27)	2.42±0.12	0.03	An aldehyde + NAD^+ + H2O = a carboxylate + NADH
13	HYOU1	Q63617	111.45	5.11	66 (17%)	9	^439DAVIYPILVEFTR^451 (30)	2.82±0.09	0.05	Has a pivotal role in cytoprotective cellular mechanisms triggered by oxygen deprivation.

^aRatios indicated the fold changes of protein volume between Tan-IIA-treated and -untreated cells for each specific protein spot. The higher ratios (>2.0) mean the proteins whose expression levels were increased upon treatment of Tan-IIA, while lower ratios (<−2.0) indicate the proteins were downregulated under the exposure to Tan-IIA.
^bP values were generated by analyzing the gel images using Prodigy SameSpots software. Differences were considered significant at P < 0.05.