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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 753971, 11 pages
Research Article

Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats

1Departamento de Farmacologia, Faculdade de Ciências Médicas, Universidade Estadual de Campinas (UNICAMP), 13083-970 Campinas, SP, Brazil
2Departamento de Biologia Estrutural e Funcional, Instituto de Biologia, UNICAMP, 13083-970 Campinas, SP, Brazil
3Departamento de Química Orgânica, Instituto de Química, UNESP, 14800-900 Araraquara, SP, Brazil
4Departamento de Morfologia, Instituto de Biociências, UNESP, 18618-970 Botucatu, SP, Brazil

Received 11 July 2012; Accepted 23 August 2012

Academic Editor: Vassya Bankova

Copyright © 2012 Felipe Meira de Faria et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (** ) and diminished the levels of TNF-α (*** ) and IL-6 (** ). BuOH fraction reduced the MPO activity (** ) and levels of TBARS (*** ); it also increased COX-1 expression, diminished the levels of TNF-α (*** ), and increased the levels of IL-12 (*** ). Besides, both treatments augmented the levels of GSH (* ), the activity of GSH-Px (** for BuOH fraction and *** for EtOAc fraction), and CAT (** ). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation.