Figure 1: Platycodi Radix activates AMPK and ACC phosphorylation in C2C12 myotubes. (a) Differentiated C2C12 myotubes were stimulated with various concentration (1, 10, 100, 400 μg/mL) of PR for 1 hr. (b) Compound C (20 μM) was pretreated for 30 min before stimulation of 10 μg/mL PR. The cell lysates were analyzed by Western blotting for pAMPK (Thr172) and pACC (Ser79). The anti-β-actin blot was visualized as an internal control.