759143.fig.002
Figure 2: Platycodi Radix inhibits adipogenic differentiation and intracellular lipid accumulation. 3T3-L1 cells were propagated to confluence and then differentiation was induced by treatment of hormone mixture (10 mg/mL of insulin, 0.5 mM of DEX, and 0.5 mM IBMX for 40 hrs and kept with 10 mg/mL of insulin and/or 10, 50, 100, 400 μg/mL PR for 7 days. Lipid accumulation was measured by Oil Red O staining. (A) Representative photomicrographs of lipid accumulation. (a) Fully differentiated cells for 7 days, (b) 200 μg/mL PR extracts treated cells, (c) 400 μg/mL PR extracts treated cells. (B) Relative intracellular lipid contents. It is considered as significant data when value is between fully differentiated cells and PR-treated groups. Data are expressed as mean SEM of triplicate experiments.; ; ; .