Figure 7: Administration of Platycodi Radix activates the ACC/AMPK phosphorylation in the muscle of obese mice. Mice were on 10% kCal ND, 60% kCal HFD, 0.1% and 1% PR in 60% kCal HFD for 10 weeks. Protein was extracted from individual frozen gastrocnemius muscles. (a) It is immunoblotted with pAMPK (Thr172) and pACC (Ser79) antibodies. The anti-β-actin blot was visualized as an internal control. (b) pAMPK and (c) pACC levels were quantitated by image densitometry and normalized by β-actin expression. ; on HFD versus WBF PR-treated group.