Research Article

7 𝜶 -Hydroxy- 𝜷 -Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation

Figure 2

Live/dead viability/cytotoxicity assay depicting the cytotoxic effects of CT1 in tumor cell lines with minimal cytotoxic effects on HMEC normal cell controls. (a) Fluorescence microscope images of viable cells stained with acetomethoxy derivate of calcein (green) and nonviable cells stained with ethidium homodimer 1 (red). (b) Percentage of viable cells as calculated under a fluorescence microscope. A total of four random quadrants were selected from each triplicate for quantification. All data were presented as mean ± SEM.
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765316.fig.002b
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