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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 765316, 12 pages
http://dx.doi.org/10.1155/2012/765316
Research Article

7 𝜶 -Hydroxy- 𝜷 -Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation

1Genetics & Molecular Biology Division, Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia
2Chemistry Department, Faculty of Science, University of Zakho, Zakho, Kurdistan Region, Iraq
3Department of Pharmacy, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia
4Centre for Natural Product Research and Drug Discovery (CENAR), Department of Chemistry, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

Received 27 April 2012; Revised 26 July 2012; Accepted 26 July 2012

Academic Editor: Olumayokun A. Olajide

Copyright © 2012 Mohammad Tasyriq et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

A complete description of the entire extraction process and identified compounds is shown. Briefly, dried ground bark of Chisocheton tomentosus (3.5 kg) was defatted with hexane and dichloromethane for five days. The crude extract was evaporated using a rotary evaporator. The extract (10.0g) was subjected to a silica gel column and eluted gradiently using hexane:DCM and DCM:acetone. Fraction 5 of DCM:acetone (60:40) was further subjected to an isocratic separation using silica gel column with acetone:DCM:hexane (25:25:50). A colorless crystal (0.2 g) of fraction 3 was obtained from the isocratic separation.

  1. Supplementary Diagram