Research Article

7 𝜶 -Hydroxy- 𝜷 -Sitosterol from Chisocheton tomentosus Induces Apoptosis via Dysregulation of Cellular Bax/Bcl-2 Ratio and Cell Cycle Arrest by Downregulating ERK1/2 Activation

Table 1

1D (1H and 13C) and 2D (HMQC and HMBC) NMR spectral data of of 7 𝛼 -hydroxy- 𝛽 -sitosterol (CT1).

Position 𝛿 H (int.; mult.; 𝐽 (Hz)) 𝛿 c HMQCHMBC

1a1.80 (1H, m)37.08Ha–C11, 3, 5, 10
1b1.01 (1H, m)Hb–C11, 3, 5, 10
2a1.80 (1H, m)31.3Ha–C21, 3
2b1.47 (1H, m)Hb–C21, 3
33.54 (1H, m)71.3H–C3
42.29 (2H, d, 5)42.0H2–C42, 5, 6, 10
5146.3
65.55 (1H, d, 5.0)123.8H–C64, 7, 8, 10
73.81 (1H, brs)65.4H–C75, 6, 9
81.43 (1H, m)37.5H–C814, 10, 4, 13, 9
91.15 (1H, m)42.3H–C98, 10, 11, 12, 19
1037.4
111.49 (2H, m)20.7H2–C119, 10, 12, 13
12a1.97 (1H, m)39.2Ha–C129, 13, 14
12b1.12 (1H, m)Hb–C129, 13, 14
1342.2
141.41 (1H, m)49.4H–C1415, 16, 17, 18
15a1.66 (1H, m)24.3Ha–C15
15b1.08 (1H, m)Ha–C15
16a1.83 (1H, m)28.3Ha–C1613, 20, 21
16b1.22 (1H, m)Ha–C1613, 20, 21
171.14 (1H, m)55.7H–C1718, 21, 15, 16
180.65 (3H, s)11.7H3–C1812, 13, 14, 17
190.95 (3H, s)19.2H3–C191, 2, 9, 10, 11
201.33 (1H, m)36.1H–C2017, 18, 23, 24
210.89 (3H, d, 6.4)18.3H3–C2117, 20, 22
222.24 (2H, m)33.8H2–C22
231.22 (2H, m)29.8H2–C2322, 24, 25
240.93 (1H, m)49.4H–C2429, 26, 27
251.64 (1H, m)29.0H–C2524, 26, 27, 28
260.81 (3H, m)19.9H3–C2624, 28
270.77 (3H, m)18.9H3–C2724, 25, 26
281.22 (2H, m)23.1H2–C2823, 24, 25, 27, 29
290.83 (3H, m)12.1H3–C2923, 24, 25, 28