Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 2

Research Article

NBM-HD-1: A Novel Histone Deacetylase Inhibitor with Anticancer Activity

Figure 2

Determination of the inhibition of total HDAC activity by NBM-HD-1. (a) C6 cells ( ) were seeded in 60 mm dishes and incubated for 14 h then treated with various concentrations (4.3–17.0 μM) of NBM-HD-1 and SB (4.0 mM, as a positive control) for 48 h, and then the nuclear fraction protein was isolated to determine the inhibition of total HDAC enzyme activity. Data are shown as the mean ± SD. * ; *** versus the control. C6 and MCF-7 cells were cultured in six-well culture plates ( /well) and treated with NBM-HD-1 (at a fixed concentration of 17.0 μM), SAHA (5.0 μM), or SB (4.0 mM) for 24 h. Immunostaining was used to evaluate biomarkers of HDACis such as Ac-histone 3 and gelsolin after cells were treated with HDACis. (b) Ac-histone 3 and (c) gelsolin protein levels were determined by confocal microscopy. All these tests were performed in three independent experiments. A representative experiment of the three replicates is shown.

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