Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 3

Research Article

NBM-HD-1: A Novel Histone Deacetylase Inhibitor with Anticancer Activity

Figure 3

Upregulation of Ac-histone 4 protein expressions after treatment with NBM-HD-1 in various cancer cell lines. (a) C6 cells, and (b) MCF-7 cells were treated with NBM-HD-1 (17.0 μM) or SB (4.0 mM) for 1-2 h or cells were treated with the same concentration of both compounds for 2 h, then the compound was removed, and cells were incubated for another 2, 4, and 6 h. Cell lysates were prepared and subjected to SDS-PAGE and immunoblotting using an Ac-histone 4 antibody.

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