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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 786487, 7 pages
http://dx.doi.org/10.1155/2012/786487
Research Article

Immunomodulatory Effect of Rhaphidophora korthalsii on Natural Killer Cell Cytotoxicity

1Institute of Bioscience, Putra University, Malaysia, Serdang, 43400 Selangor, Malaysia
2Department of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Putra University, Malaysia, Serdang, 43400 Selangor, Malaysia
3Faculty of Agriculture and Biotechnology, Sultan Zainal Abidin University, Malaysia (UniSZA), Kota Campus, Jalan Sultan Mahmud, 20400 Kuala Terengganu, Malaysia
4Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Putra University, Malaysia, Serdang, 43400 Selangor, Malaysia
5Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Putra University, Malaysia, Serdang, 43400 Selangor, Malaysia

Received 28 April 2011; Revised 15 July 2011; Accepted 16 July 2011

Academic Editor: Alfredo Vannacci

Copyright © 2012 Swee Keong Yeap et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The in vivo immunomodulatory effect of ethanolic extracts from leaves of Rhaphidophora korthalsii was determined via immune cell proliferation, T/NK cell phenotyping, and splenocyte cytotoxicity of BALB/c mice after 5 consecutive days of i.p. administration at various concentrations. Splenocyte proliferation index, cytotoxicity, peripheral blood T/NK cell population, and plasma cytokine (IL-2 and IFN-) in mice were assessed on day 5 and day 15. High concentration of extract (350 μg/mice/day for 5 consecutive days) was able to stimulate immune cell proliferation, peripheral blood NK cell population, IL-2, and IFN- cytokines, as well as splenocyte cytotoxicity against Yac-1 cell line. Unlike rIL-2 which degraded rapidly, the stimulatory effect from the extract managed to last until day 15. These results suggested the potential of this extract as an alternative immunostimulator, and they encourage further study on guided fractionation and purification to identify the active ingredients that contribute to this in vitro and in vivo immunomodulatory activity.