In Vivo Anti-Influenza Virus Activity of Japanese Herbal (Kampo) Medicine, “Shahakusan,” and Its Possible Mode of Action
Effects of SHS on IL-4, IL-1β, IFN-γ, IL-10, IL-12A, and IL-12B mRNA expressions in the mandibular lymph nodes of influenza virus infected mice (upper respiratory tract infection). BALB/c mice were administered orally with water or SHS from one day before to 4 days p.i. (−1 d to 4 d), or 2 hours p.i. to 4 days p.i. (2 h to 4 d), as shown in Figure 2(a). The mRNA expressions in the mandibular lymph nodes of influenza virus infected mice were estimated at 5 day p.i. and those of mock-infected mice were estimated at next day after 5 days administration (same duration from 2 hours p.i. to 4 days p.i.) of water or SHS with real-time PCR. Values represent means ± S.E. ().