Mechanisms of Edible Bird's Nest Extract-Induced Proliferation of Human Adipose-Derived Stem Cells
Figure 1
Effects of EBNE on the proliferation of hADSCs, NHFs, Hep-3B, and MCF-7. (a)–(d) Cells were cultured in serum-free DMEM in the presence or absence of the indicated concentrations of EBNE. Cell proliferation was then determined using the MTT assay. (a), (b), (c), and (d) represent the proliferation of hADSCs, NHFs, Hep-3B, and MCF-7, respectively. For the positive control, cells were cultured in control medium (DMEM + 15% FBS: hADSCs, Hep-3B, MCF-7 and NHFs; DMEM + 10% FBS). Values are represented as percentage compared with the control. Results are shown as mean ± SD (). * versus serum-free control. Data are representative of at least three independent experiments. (e)–(h) Cell proliferation was determined using Click-iT EdU flow cytometry. (e), (f), (g), and (h) represent the proliferation of hADSCs, NHFs, Hep-3B, and MCF-7, respectively. Serum (−): serum-free, EBNE: 2,000 ppm of EBNE. Data are representative of at least three independent experiments.
