Research Article

Anti-Inflammatory Effect of Triterpene Saponins Isolated from Blue Cohosh (Caulophyllum thalictroides)

Figure 4

Effects of blue cohosh constituents on the LPS-induced expressions of TNF-α and IL-6 expression in BV2 cells. Microglial cells were treated by applying the blue cohosh constituents with 100 ng/mL LPS for 24 h. Expression of TNF-α and IL-6 was measured by immunoblot analysis. Blue cohosh suppressed the TNF-α and IL-6 in LPS-activated microglia, respectively. GAPDH was used as an internal control. Cell extracts were collected from cultured microglia after activation by LPS with cotreatment of blue cohosh, and immunoblot analysis was performed using TNF-α and IL-6 antibodies. Blue cohosh inhibited the activation of TNF-α and IL-6 at different dose. All values are expressed as mean ± S.E.M. from three independent experiments. Data were analyzed by one-way ANOVA for multiple comparison and Student-Newman-Keuls test as post hoc test. # 𝑃 < 0 . 0 1 in comparison with sham, * 𝑃 < 0 . 0 1 in comparison with LPS.
798192.fig.004a
(a) T N F - 𝛼
798192.fig.004b
(b) IL-6