Inhibition of Activity of GABA Transporter GAT1 by -Opioid Receptor
Effects of OR coexpression and its activation by 100 nM DPDPE on GAT1-mediated transient charge movements. 40 ng of GAT1 cRNA alone or 40 ng of GAT1 and 20 ng of OR cRNA were injected into oocytes. (a) Voltage dependencies of moved charge in response to rectangular potential jumps were obtained by integration of the respective transient currents and were fitted by (2) (see lines). (b) The rate constants were obtained by fitting an exponential to the transient current signal, and the voltage dependencies were fitted by (3) (see lines). Data represent means SEM; = 6-7 oocytes for each group.