Inhibition of Activity of GABA Transporter GAT1 by -Opioid Receptor
GAT1 expression in Xenopus oocyte membrane fractions. (a) Lane 1: noninjected oocytes, lane 2: oocytes injected with 40 ng of GAT1 cRNA and 20 ng of OR, and lane 3: oocytes injected with 40 ng of GAT1 cRNA alone. The bands at about 60 kDa (see arrow) represent GAT1 monomers. (b) GAT1 monomer band intensities averaged from 5 batches of oocytes and normalized to the respective batch injected with cRNA for GAT1 only.