Regulation of Proinflammatory Mediators via NF-κB and p38 MAPK-Dependent Mechanisms in RAW 264.7 Macrophages by Polyphenol Components Isolated from Korea Lonicera japonica THUNB
Figure 3
Effect of polyphenols on iNOS mRNA and protein expressions in RAW 264.7 macrophages. RAW 264.7 cells were pretreated with the various concentrations of polyphenols for 1 h and treated with LPS (1 μg/mL). (a) After 6 h, total RNA of the cells was subjected to RT-PCR. (b) After 24 h, equal amounts of protein were subjected to 10% SDS-PAGE. Expression of COX-2 proteins was determined by Western blot analysis. Data are the mean ± SD of triplicates. The asterisk (*) indicates a significant difference from the control group () and **indicates significant difference from the LPS-treated group ().
