Regulation of Proinflammatory Mediators via NF-κB and p38 MAPK-Dependent Mechanisms in RAW 264.7 Macrophages by Polyphenol Components Isolated from Korea Lonicera japonica THUNB
Figure 5
Effect of polyphenols on activation of NF-κB in cytosol and nuclear translocation of NF-κB p65. The cells were pretreated with the indicated concentrations of polyphenols for 1 h and treated with LPS (1 μg/mL). After 30 min incubation, the expression of (a) cytosolic and (b) nucleic NF-κB as well as (c) IκB and (d) phosphorylated IκB was examined by Western blot. Data are the mean ± SD of triplicates. The asterisk (*) indicates a significant difference from the control group () and **indicates significant difference from the LPS-treated group ().
