Epigenetic Changes in Response to Tai Chi Practice: A Pilot Investigation of DNA Methylation Marks

Table 3

Genetic loci and primer sequences used for DNA methylation analysis.

Genetic locus	Primer	Primer sequence	Product size; (bp)	Physical location	No. of CpGs
Rad 50	Forward	GGAGTTTTAGGATTAAGTTTTGGGA	467	Chr5: 131920312–131920779	18
	Reverse	CTACCAAAAAATCCTACCAACCAA
17p (subtel)	Forward	AGTGAGGAAGAGGTTTTTGGTTTAT	229	Chr17: 2774–3003	8
	Reverse	ACTAAACCCCTACAACTAAATCCCA
G6PD	Forward	GGAAAAGTTGAGGTATGGAGTAGGT	378	ChrX: 153427381–153427759	8
	Reverse	TAAAACCCAAAACCAACAATTTCTA
Xp13 (subtel)	Forward	TGGGTTTAGGTGTTATTGAGGATAG	309	ChrX: 34124–34433	2
	Reverse	CCCAATAAAAACCCTAAAAATTACA
Ercc1	Forward	TGTGTGTTTAGAATTTTGGTAGGGT	413	Chr19: 50619182–50619595	10
	Reverse	CTACTCAAATATCCCTTCCTCCAA
Esr 1	Forward	TGGTAGGTTGTATTTTTTTGATGGT	265	Chr6: 152169890–152170155	4
	Reverse	ATTTATCTCTCTTTCTATTTAATTCCCCC
WRN	Forward	TTTGTTTTTAGGATATTGTGAGGATG	444	Chr8: 31009321–31009765	10
	Reverse	CAAAAATAAACTCTATCCCCAAACC