Identification of Major Active Ingredients Responsible for Burn Wound Healing of Centella asiatica Herbs

Figure 3

Effects of asiaticoside (AD), madecassoside (MD), asiatic acid (AA), and madecassic acid (MA) on collagen synthesis in human skin fibroblasts. The cells were treated with various concentrations of the four triterpene compounds for 24 h. ((a)–(c)) The mRNA expressions of collagen type I and type III were determined via RT-PCR assay. ((d), (e)) The cell supernatants were prepared and analyzed for protein expressions of procollagen I N-terminal peptide (PINP) and procollagen III N-terminal peptide (PIIINP) by ELISA. Each column represents the mean ± S.D. from three independent experiments. *𝑃<0.05, **𝑃<0.01 versus control.

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