Research Article
Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset
Table 1
List of 32 acupuncture-specific differentially expressed proteins in asthma onset.
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The 32 acupuncture-specific differentially expressed protein spots in asthma onset can be divided into six different categories according to their expression patterns on 2DE gels (NC: normal control, AS: asthma model, AA: asthma model treated with acupuncture, and NA: normal rats treated with acupuncture). Patterns I and II, proteins that exhibited either increased or decreased levels in AS compared with NC that were restored to normal expression levels by acupuncture; Patterns III and IV, proteins that did not exhibit changed levels in AS compared with NC but did exhibit either increased or decreased levels after acupuncture; Patterns V and VI, proteins that exhibited either increased or decreased levels in AS compared with NC and exhibited similar expression patterns after acupuncture in AA compared with NC. aThe spot number from 2DE gel analysis (Figure 3); bthe number of matching peptides (Pept.) and percentage of the total amino acid sequence covered by the peptides (% Seq.Cov) from the mass mapping experiments; cprotein spot 24 is actually the soluble receptor for advanced glycation end products (sRAGE), which corresponds to the extracellular domain of full-length RAGE lacking the cytosolic and transmembrane domains, as revealed by BLAST and verified by western blot analysis; dspot not detected in gels from the NC and NA samples; espot not detected in gels from the NC samples; fspot not detected in gels from the AS samples; gspot not detected in gels from the AA samples; hspot detected only in the AA samples. |