Research Article

Proteomic Analysis Reveals the Deregulation of Inflammation-Related Proteins in Acupuncture-Treated Rats with Asthma Onset

Table 1

List of 32 acupuncture-specific differentially expressed proteins in asthma onset.

Spot no.aProtein nameGene symbolSwiss-Prot no.Expression level ratios Theoretical Mr(kDa)/PI
Pept./cov %b

Pattern I of changes in expression 850512.tab.001a   

1Protein S100-A8S100a8P501154.449 0.553 0.951 10.2/5.76/56.18%
2Protein S100-A8S100a8P5011513.674 0.464 1.669 10.2/5.726/41.57%
3Similar to destrinLOC296197XP_215862d0.158 d18.5/7.511/27.88%
4Actin, cytoplasmic 1 ActbP607112.882 0 g4.168 41.7/5.313/10.67%
5Rho, GDP dissociation inhibitor (GDI) betaArhgdibQ5M860d0.101 d22.9/5.04/21.00%
6Actin, alpha skeletal muscle Acta1P6813613.250 0.145 4.582 42.0/5.28/11.94%
7Serum albumin precursor AlbP02770e0.231 e68.7/6.17/6.58%
8ATP synthase subunit alpha, mitochondrial precursorAtp5a1P15999d0.197 d59.8/6.253/22.24%
9Glyceraldehyde-3-phosphate dehydrogenaseGapdhP04797d0.203 d35.8/8.15/12.31%
10Glyceraldehyde-3-phosphate dehydrogenaseGapdhP047973.905 0.248 0.828 35.8/8.117/29.73%
11Anionic trypsin-1 precursorPrss1P00762d0.420 d26.0/4.74/8.13%
1222 kDa protein2.288 0.442 0.591 6/16.83%
13Na+/ -transporting ATPase subunit alpha-1 precursorAtp1a1P06685d0.471 d11.3/5.316/13.29%
14Tubulin alpha-1A chainTuba1aP68370d0.425 d50.1/4.911/13.53%
15Phosphoglycerate kinase 1Pgk1P166173.728 0.369 1.121 44.5/8.09/29.50%
16S100a11S100a11Q6B3454.190 0 g1.087 11.1/5.63/26.50%
17Similar to Ras suppressor protein 1Rsu1D3ZCX02.211 0.429 1.264 31.5/8.54/10.83%

Pattern II of changes in expression 850512.tab.001b     

18Uteroglobin precursor Scgb1a1P175590.145 3.830 0.502 10.4/4.97/25.00%
19N-acetylneuraminic acid synthaseNansB1WC260.281 2.049 1.147 40.0/6.49/17.83%
20Bifunctional purine biosynthesis protein PURHAticO355670 ff1.623 64.2/6.75/6.42%
21Creatine kinase B-typeCkbP073350 ff1.871 42.7/5.42/4.46%
22Carbonic anhydrase 2Ca2P271390.227 3.436 1.031 29.1/6.932/50.38%
2322 kDa protein0.129 5.823 1.782 5/22.12%
24Advanced glycosylation end product-specific receptorcAgerQ634950.095 3.334 1.035 42.7/5.825/23.38%
25Similar to proteasome 26S ATPase subunit 6Psmc6Q32PW90 ff1.152 45.8/7.617/27.79%
26Cofilin-1Cfl1P455920ff0.541 18.5/8.295/78.92%
27Heat shock-related 70 kDa protein 2Hspa2P146590.124 2.798 0.660 69.6/5.56/4.27%
Pattern III of changes in expression 850512.tab.001c     

28Annexin A5Anxa5P14668hhh35.7/4.914/23.20%

Pattern IV of changes in expression 850512.tab.001d     

29Peroxiredoxin-6Prdx6O352441.245 0.263 1.018 24.8/5.613/26.34%

Pattern V of changes in expression 850512.tab.001e     

30Gamma-enolase Eno2P073230.280 0 g0.553 47.0/7.02/5.07%
31Protein disulfide isomerase associated 6 Pdia6Q630810.311 0.413 1.224 48.8/5.020/26.07%

Pattern VI of changes in expression 850512.tab.001f     

32MyotrophinMtpnP62775d2.054 d12.9/5.36/25.42%

The 32 acupuncture-specific differentially expressed protein spots in asthma onset can be divided into six different categories according to their expression patterns on 2DE gels (NC: normal control, AS: asthma model, AA: asthma model treated with acupuncture, and NA: normal rats treated with acupuncture). Patterns I and II, proteins that exhibited either increased or decreased levels in AS compared with NC that were restored to normal expression levels by acupuncture; Patterns III and IV, proteins that did not exhibit changed levels in AS compared with NC but did exhibit either increased or decreased levels after acupuncture; Patterns V and VI, proteins that exhibited either increased or decreased levels in AS compared with NC and exhibited similar expression patterns after acupuncture in AA compared with NC.
aThe spot number from 2DE gel analysis (Figure 3); bthe number of matching peptides (Pept.) and percentage of the total amino acid sequence covered by the peptides (% Seq.Cov) from the mass mapping experiments; cprotein spot 24 is actually the soluble receptor for advanced glycation end products (sRAGE), which corresponds to the extracellular domain of full-length RAGE lacking the cytosolic and transmembrane domains, as revealed by BLAST and verified by western blot analysis; dspot not detected in gels from the NC and NA samples; espot not detected in gels from the NC samples; fspot not detected in gels from the AS samples; gspot not detected in gels from the AA samples; hspot detected only in the AA samples.