Xanthohumol, a Prenylated Flavonoid from Hops (Humulus lupulus), Prevents Platelet Activation in Human Platelets

Figure 3

Inhibitory activity of xanthohumol (XN) on protein kinase C (PKC) activation in activated platelets. Washed platelets were preincubated with 1.5 and 3 μM XN or 0.5% DMSO, followed by the addition of 1 μg/mL collagen or 150 nM PDBu to trigger ((a) and (b)) PKC activation or (c) platelet aggregation. Cells were collected, and subcellular extracts were analyzed for ((a) and (b)) phosphorylation of the PKC substrate (p-p47) as described in Section 2. Data ((a) and (b)) are presented as the means ± S.E.M. (𝑛=5). **𝑃<0.01 and ***𝑃<0.001 compared to the resting group; ^#𝑃<0.05 and ^##𝑃<0.01 compared to the control (DMSO) group. Profiles (c) are representative examples of three similar experiments.

(a)

(b)

(c)