Research Article

Dentatin Induces Apoptosis in Prostate Cancer Cells via Bcl-2, Bcl-xL, Survivin Downregulation, Caspase-9, -3/7 Activation, and NF-κB Inhibition

Figure 5

Dentatin mediates antiproliferative effect and apoptosis through induction of oxidative stress. ROS production in dentatin-treated PC-3 and LNCaP cells after 24 h was detected with DCFH-DA dye. DCFH-DA is rapidly oxidized to DCF by ROS and fluorescence can be measured with a fluorescent microplate reader. The levels of DCF fluorescence in dentatin-treated cells were markedly increased. Data were shown as mean ± SD. Significant differences between dentatin-treated and untreated control cells.
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