Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 3

Research Article

Aqueous Extract of Solanum nigrum Leaf Activates Autophagic Cell Death and Enhances Docetaxel-Induced Cytotoxicity in Human Endometrial Carcinoma Cells

Figure 3

Detection of cell death markers in AE-SN-treated human endometrial cancer cells. (a) HEC1A, HEC1B and KLE cells were treated with 0, 0.5, and 1.0 mg/mL AE-SN for 48 hr, and total protein extracts were harvested for western blotting analysis of the cell death markers, PARP, caspase-3 and LC3 A/B. (b) Accumulation of LC3 A/B II was semiquantified by Image J software. All experiments were replicated five times for statistical analysis of semiquantified data and are presented as . *Indicates statistical significance compared with 0 mg/mL AE-SN treatment by Student’s -test ( ).

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