Towards Modernization of the Formulation of the Traditional Uighur Medicine Herbal Preparation Abnormal Savda Munziq
Figure 3
Dose-response curves of antiproliferative activity of Extr-4 after 24 h (a), 48 h (b), and 72 h (c). HL-60 cells were cultured in 12-well plates for 24 h to reach logarithmic growth phase and then incubated with 25–250 μg/mL Extr-4. Cell viability was estimated by tryptan blue staining and cell counting after 24 h, 48 h, and 72 h. Data are presented as percentage of control value. The horizontal axis represents a log scale of concentration of Extr-4. The IC50 was calculated using a sigmoidal dose-response model with variable slope. The curve represents the average of experiments performed in triplicate. *, compared with respective control value (24 h : 100.00 ± 8.50%; 48 h : 100.00 ± 8.84%, 72 h : 100.00 ± 22.26%).
