Research Article

HRT, Herbal Formula, Induces G2/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells

Figure 1

HPLC fingerprints of HRT. (a) HPLC profiling of standard components of constituent herbs contained in HRT. (b) Identification of components in HRT prescription by HPLC. HPLC chromatogram of components was monitored at 230 nm. The geniposide, palmatine-HCl, berberine-HCl, and baicalein were detected and determined as a constituent of G. jasminoides, P. amurense, C. japonica, and S. baicalensis, respectively. The retention times of standards for the four constituent herbs were detected at 4.93, 28.54, 30.01, and 40.76 min. Four components in HRT were detected at 4.88, 28.92, 30.18, and 40.71 min.
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(a)
871893.fig.001b
(b)