HRT, Herbal Formula, Induces G2/M Cell Cycle Arrest and Apoptosis via Suppressing Akt Signaling Pathway in Human Colon Cancer Cells
Figure 3
The effect of HRT on cell cycle progression in HCT116 cells. (a) The cells were treated with HRT (300 μg/mL) for 12 h, 24 h, and 48 h. After fixing in 70% ethanol, the cells were stained with propidium iodide (PI) at 37°C for 30 min and analyzed by a flow cytometric system. (b) Histogram represents cell cycle analysis on HCT116 cells. *, #, and ‡ mean respective P value versus CTL. (c) Expression of cell cycle regulatory proteins in HRT-treated cells. HRT (100 or 300 μg/mL) was treated in HCT116 cells for 24 h. Western blot analyses were done with anti-cyclin D1, -cyclin B1, -CDK7, and GAPDH antibodies.
