Evidence-Based Complementary and Alternative Medicine / 2012 / Article / Fig 4

Research Article

Active Hydrophilic Components of the Medicinal Herb Salvia miltiorrhiza (Danshen) Potently Inhibit Organic Anion Transporters 1 (Slc22a6) and 3 (Slc22a8)

Figure 4

determination for LSA, RMA, and SAA in the CHO-mOat1 cell line. Two minute uptake of [3H]PAH (5 μM) in CHO-mOat1 cells was measured in the presence of increasing concentrations (10−7 to 5 × 10−4 M) of LSA, RMA, and SAA. Data were corrected for nonspecific background measured in the CHO-FRT cells prior to kinetic analysis. values were determined with non-linear regression and the “one-site competition” model using GraphPad Prism software. Experiments were repeated three times in triplicate with the mean ± S.E.M. reported in Table 2. Graphs shown are from representative experiments with values plotted as mean ± S.D. ( ).
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