Effects of Extracts from Trifolium medium L. and Trifolium pratense L. on Development of Estrogen Deficiency-Induced Osteoporosis in Rats

Figure 1

Effects of Trifolium medium L. (TM) and Trifolium pratense L. (TP) extracts on mechanical properties of the femoral diaphysis (the maximum load point) in ovariectomized (OVX) rats. The lyophilized extracts from TM or TP at doses corresponding to 10 and 20 mg/kg of isoflavone aglycones daily (TM10, TP10 and TM20, TP20, resp.), or estradiol (0.2 mg/kg daily), were administered orally to OVX rats for 4 weeks. Results are presented as the mean ± SEM (𝑛=9-10). One-way ANOVA followed by Duncan’s test was used for evaluation of the significance of the results. *𝑃<0.05—significantly different from sham-operated control rats (SHAM). ^•𝑃<0.05, ^••𝑃<0.01—significantly different from OVX control rats.

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