Antiviral Activity of Isatis indigotica Extract and Its Derived Indirubin against Japanese Encephalitis Virus

Table 1

Cytotoxic effect of I. indigotica extracts and its marker components on BHK-21 and HL-CZ cells.

Isatis  indigotica	CC50  (𝜇g/mL)
	BHK-21^a	HL-CZ^a
Ethyl acetate extract	>100	49.02±2.04
Methanol extract	>100	>100
Indigo	86.88±3.72	99.41±0.54
Indirubin	>100	64.89±1.08
Tryptathrin	4.57±0.26	1.74±0.34
Adenosine	24.10±2.57	15.38±1.05
Betulin	13.30±2.58	2.10±0.78
Lupeol	9.53±1.32	3.44±1.17
2-Benzoxazolinone	19.92±1.57	67.33±10.2

^aMeasured using trypan blue staining.