Effects of a Chinese Herbal Medicine, Guan-Jen-Huang (Aeginetia indica Linn.), on Renal Cancer Cell Growth and Metastasis
Figure 2
Synergistic effect between GJH and 5-FU on 786-O cancer cell apoptosis. (a) A dose-response survival curve for GJH and the chemotherapeutic agent 5-FU in 786-O cells. (b) CI values at different levels of growth inhibition effect (fa). (c) GJH (CC12.5 and CC25) enhances the apoptotic response to 5-FU (CC12.5 and CC25, resp.) as demonstrated by Annexin V-binding assay. (d) Combination treatment with GJH and 5-FU upregulates the expression of ERCC1 but downregulates the expression of TUBB3, Tau, and TS1 as shown by real-time quantitative RT-PCR. (e) Western blot analysis indicates that GJH (CC25) enhances the 5-FU (CC25)-induced cleavage of PARP, as well as 5-FU-induced reduction in expression of -catenin and cyclin D1. The mean (SD) is shown from at least 3 separate experiments.
