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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 942384, 6 pages
Research Article

UPLC-QTOF/MS Analysis of Alkaloids in Traditional Processed Coptis chinensis Franch.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China

Received 16 August 2012; Revised 29 November 2012; Accepted 29 November 2012

Academic Editor: Muhammad Nabeel Ghayur

Copyright © 2012 Xue Jiang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The processing technology employed in traditional Chinese medicine (TCM) is significant and distinct. Meanwhile, the processed Coptis chinensis Franch. are significant in clinic based on clinical practice and literature. The current study used ultraperformance liquid chromatography method (UPLC) coupled with quadrupole time of flight mass spectrometry (qTOF/MS) and Marklynx software to analyze the chemical profiles of crude and processed C. chinensis Franch. 13 compounds in these samples are identified, including 3 compounds that are detected in C. chinensis Franch. for the first time. Moreover, the results of the experiment show significant chemical differences between crude and processed C. chinensis Franch. with principal component analysis (PCA). The obvious separation in PCA confirms the traditional processing theory in TCM.