TY - JOUR A2 - De Feo, Vincenzo AU - Takemura, Tomoaki AU - Urushisaki, Tomohiko AU - Fukuoka, Mayuko AU - Hosokawa-Muto, Junji AU - Hata, Taketoshi AU - Okuda, Yumiko AU - Hori, Sachie AU - Tazawa, Shigemi AU - Araki, Yoko AU - Kuwata, Kazuo PY - 2012 DA - 2011/08/25 TI - 3,4-Dicaffeoylquinic Acid, a Major Constituent of Brazilian Propolis, Increases TRAIL Expression and Extends the Lifetimes of Mice Infected with the Influenza A Virus SP - 946867 VL - 2012 AB - Brazilian green propolis water extract (PWE) and its chemical components, caffeoylquinic acids, such as 3,4-dicaffeoylquinic acid (3,4-diCQA), act against the influenza A virus (IAV) without influencing the viral components. Here, we evaluated the anti-IAV activities of these compounds in vivo. PWE or PEE (Brazilian green propolis ethanol extract) at a dose of 200 mg/kg was orally administered to Balb/c mice that had been inoculated with IAV strain A/WSN/33. The lifetimes of the PWE-treated mice were significantly extended compared to the untreated mice. Moreover, oral administration of 3,4-diCQA, a constituent of PWE, at a dose of 50 mg/kg had a stronger effect than PWE itself. We found that the amount of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) mRNA in the mice that were administered 3,4-diCQA was significantly increased compared to the control group, while H1N1 hemagglutinin (HA) mRNA was slightly decreased. These data indicate that PWE, PEE or 3,4-diCQA possesses a novel and unique mechanism of anti-influenza viral activity, that is, enhancing viral clearance by increasing TRAIL. SN - 1741-427X UR - https://doi.org/10.1155/2012/946867 DO - 10.1155/2012/946867 JF - Evidence-Based Complementary and Alternative Medicine PB - Hindawi Publishing Corporation KW - ER -