Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF-𝜷1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

Table 1

The changes of Col. I in LX-2 cell culture supernatants following inhibition of ERK and Smad4 signaling pathways.

Group	𝑛	mean ± S
Control	3	0.567 ± 0.093
Negative control	3	0.469 ± 0.004
SRV4	3	0.351 ± 0.011^##
TGF	3	1.007 ± 0.017^##
SM4 + TGF	3	0.648 ± 0.012^▲▲
SM4 + TGF + SA-B	3	0.289 ± 0.022
SM4 + TGF + PD	3	0.294 ± 0.023**
TGF + SA-B + PD	3	0.439 ± 0.003
SM4 + TGF + SA-B + PD	3	0.252 ± 0.004^♦♦

The levels of Col. I protein were determined by ELISA kit as indicated. Results were expressed as mean ± SE of three independent experiments performed in triplicate. ^##Significant difference versus Control, Negative control, and SRV4 (𝑛=3, 𝑃<0.01); ^▲▲Significant difference versus SRV4 and TGF (𝑛=3, 𝑃<0.01); **Significant difference versus SM4 + TGF (𝑛=3, 𝑃<0.01); ^♦♦Significant difference versus SM4 + TGF + SA-B, SM4 + TGF + PD, and TGF + SA-B + PD (𝑛=3, 𝑃<0.01).