Hochuekkito (TJ-41), a Kampo Formula, Ameliorates Cachexia Induced by Colon 26 Adenocarcinoma in Mice

Figure 2

Effect of hochuekkito on tumor growth (a), carcass weight (b), food intake (c), and water intake (d) in colon 26/clone 20 adenocarcinoma-bearing mice. Tumor cells (1 × 10⁶) were implanted s.c. into mice on day 0, and hochuekkito administered orally to mice for consecutive 15 days (⚪ tumor (−), hochuekkito (+); 𝑛=5, ■ tumor (+), hochuekkito (+); 𝑛=4) and no drug was given to another group (▲ tumor (+), hochuekkito (−); 𝑛=4). The group tumor (−), hochuekkito (−) was healthy control mice (◆; 𝑛=5). Tumor size, body weight, food intake, and water intake were measured on days 0, 3, 7, 10, and 14. The measured quantity of food or water was divided by the number of mice and days to determine each intake per animal per day. Animal experiments were performed twice, and a representative result is depicted. Data are presented as means ± SD. Statistical significance was evaluated with Tukey’s Honest Significant Differences (HSD) after the one-way ANOVA. ^‡No significant difference in tumor growth between hochuekkito (−) or hochuekkito (+) on day14. *Significantly different from the group of tumor (−), hochuekkito (−) in the same day; 𝑃<0.01. ^†Significantly different from the group of tumor (+), hochuekkito (−) in the same day; 𝑃<0.01. ^††Significantly different from the group of tumor (+), hochuekkito (−) in the same day; 𝑃<0.05.

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