Cardioprotective Effects of Qishenyiqi Mediated by Angiotensin II Type 1 Receptor Blockade and Enhancing Angiotensin-Converting Enzyme 2

Table 1

Nucleotide sequences of primers used in real-time PCR.

Gene (accession no.)	Primers	Nucleotide sequence 5′-3′	Size (bp)	Temp. (°C)
ACE	Forward	GTCCTATTCCCGCTCATCT	128	53.1
	Reverse	CCAGCCCTTCTGTACCATT
ACE2	Forward	AGAATGCGACCATCAAGCG	230	52.6
	Reverse	AAGCCCAGAGCCTACGATT
GAPDH	Forward	CACTGCCACTCAGAAGACT	177	53.5
	Reverse	ACGTTGGGGGTAGGAACAC