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Evidence-Based Complementary and Alternative Medicine
Volume 2012, Article ID 980276, 10 pages
Research Article

Rutin, a Flavonoid That Is a Main Component of Saussurea involucrata, Attenuates the Senescence Effect in D-Galactose Aging Mouse Model

1Department of Biotechnology and Animal Science, College of Bioresources, National Ilan University, Ilan 260, Taiwan
2Laboratory of Exercise Biochemistry, Taipei Sports University, Taipei 111, Taiwan
3Institute of Statistical Science, Academia Sinica, Taipei 115, Taiwan
4NTU Center for Genomic Medicine, National Taiwan University College of Medicine, Taipei 100, Taiwan
5The Ph.D. Program for Cancer Biology and Drug Discovery, China Medical University, Taichung 404, Taiwan
6Graduate Institute of Cancer Biology and Center for Molecular Medicine, China Medical University, Taichung 404, Taiwan
7Department of Biotechnology, Asia University, Taichung 413, Taiwan
8Department of Clinical Laboratory Sciences and Medical Biotechnology, College of Medicine, National Taiwan University, Taipei 100, Taiwan
9Department of Laboratory Medicine, National Taiwan University Hospital, Taipei 100, Taiwan
10Sensory Physiology Laboratory, Institute of Cellular and Organismic Biology, Academia Sinica, Jiaoshi, Ilan 262, Taiwan

Received 5 March 2012; Accepted 19 June 2012

Academic Editor: Yukihiro Shoyama

Copyright © 2012 Ying-Chen Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Saussurea involucrata (Kar. et Kir.), known as the snow lotus, grows in the Tian Shan and A’er Tai areas of China. It has recently been reported that the ethyl acetate extract of S. involucrata (SI-2) can inhibit proliferation and induce apoptosis in PC-3 human prostate cancer cells. This study investigated the protective effect of ethyl acetate extract of S. involucrata (SI-2) or rutin, a flavonoid extracted from ethyl acetate extract of S. involucrata (SI-2), on D-galactose- (D-gal-) induced brain injury in mice. Administering SI-2 or rutin (30 mg/kg/d and 30 mg/kg/d) for 6 weeks, concomitant with D-gal injection, significantly increased superoxide dismutase and glutathione peroxidase activities and decreased the MDA level in plasma. Furthermore, the result showed that the percentages of cleaved caspase-3 and PARP in the D-gal-treated mice were much higher than those in the control. Pretreatment using SI-2 or rutin decreased the expression of cyclooxygenase-2 via downregulation of NF-kappaB, resulting in a decrease in lipid peroxidation. Furthermore, our results also showed that oral administration of rutin to these mice significantly improved behavioral performance in a step-through passive avoidance task and these results suggest that SI-2 or rutin exerts potent antiaging effects on D-gal in mice via antioxidative mechanisms.