Epimedium koreanum Nakai Water Extract Exhibits Antiviral Activity against Porcine Epidermic Diarrhea Virus In Vitro and In Vivo

Table 1

Screening of herbal extract containing antiviral activity against PED virus. 333 herbal extracts were evaluated for the inhibitory effect on PED viral production and 27 herbal extracts having anti-PED viral activity were selected. Antiviral activity was examined by plaque assay and determined as a PFU (plaque forming unit). KIOM 124 and KIOM 198 exhibited strong inhibitory effect on PED viral propagation.

Extract number	PEDV-PFU	PEDV log PFU	Fold inhibition
30	713.1	2.853	410
32	7391	3.869	40
43	2488	3.396	118
44	1736	3.24	168
48	2962	3.472	99
49	20570	4.313	14
51	85600	4.932	3
55	1084	3.035	270
56	2174	3.337	135
57	2678	3.428	109
58	1817	3.259	161
60	6042	3.781	48
71	256.8	2.41	1139
77	749.5	2.875	390
78	47.48	1.676	6160
124	<1	<0	>292500
130	133.9	2.127	2184
143	179100	5.253	2
198	<1	<0	>292500
208	2563	3.409	114
223	58240	4.765	5
232	2212	3.345	132
274	344.2	2.537	850
297	431900	5.635	1
324	3582	3.554	82
325	734200	5.866	No inhibition
326	165600	5.219	2
Positive control (No treatment)	292500	5.466