Evaluation of Acute 13-Week Subchronic Toxicity and Genotoxicity of the Powdered Root of Tongkat Ali (Eurycoma longifolia Jack)

Table 6

The absolute and relative tissue weights of subchronic toxicity after 13-week E. longifolia powdered root treatment.

		Treatment (g/kg b.w.)
		Male				Female
		0	0.6	1.2	2	0	0.6	1.2	2
Brain
Absolute weight	Gram (10^−3)	2.16±0.11	2.18±0.10	2.17±0.10	2.10±0.16	2.06±0.06	2.03±0.11	2.04±0.06	2.07±0.07
Ratio per body weight		4.83±0.50	4.87±0.37	4.95±0.53	4.78±0.55	7.63±0.43	7.54±0.68	7.76±0.90	7.79±0.60
Adrenals
Absolute weight	Gram (10^−3)	0.02±0.00	0.03±0.00	0.02±0.01	0.03±0.00	0.03±0.01	0.03±0.00	0.04±0.01	0.03±0.01
Ratio per body weight		0.05±0.01	0.06±0.01	0.05±0.01	0.06±0.01	0.13±0.02	0.12±0.02	0.14±0.04	0.12±0.04
Ratio per brain		0.01±0.00	0.01±0.00	0.01±0.00	0.01±0.00	0.02±0.00	0.02±0.00	0.02±0.00	0.02±0.0
Heart
Absolute weight	Gram (10^−3)	1.19±0.17	1.19±0.10	1.19±0.13	1.27±0.15	0.82±0.07	0.83±0.06	0.82±0.11	0.82±0.07
Ratio per body weight		2.63±0.33	2.66±0.21	2.70±0.25	2.87±0.27	3.06±0.29	3.07±0.22	3.12±0.40	3.05±0.19
Ratio per brain		0.55±0.08	0.55±0.05	0.55±0.06	0.60±0.06	0.40±0.04	0.41±0.03	0.41±0.06	0.40±0.04
Kidneys
Absolute weight	Gram (10^−3)	1.18±0.08	1.22±0.08	1.19±0.18	1.19±0.08	0.83±0.05	0.86±0.11	0.86±0.14	0.76±0.07
Ratio per body weight		2.63±0.23	2.73±0.20	2.69±0.16	2.72±0.27	3.08±0.22	3.20±0.47	3.22±0.39	2.86±0.17
Ratio per brain		0.55±0.04	0.56±0.04	0.55±0.08	0.57±0.06	0.40±0.02	0.42±0.04	0.42±0.07	0.37±0.04
Liver
Absolute weight	Gram (10^−3)	10.82±1.23	10.84±1.19	9.91±1.37	10.02±0.96	7.70±1.00	7.89±1.30	7.04±1.18	7.05±0.74
Ratio per body weight		24.03±2.26	24.10±1.62	22.37±1.28	22.77±2.40	28.48±2.72	29.28±5.06	26.47±3.01	26.44±2.18
Ratio per brain		5.02±0.62	4.98±0.59	4.57±0.62	4.80±0.57	3.75±0.46	3.89±0.66	3.46±0.60	3.42±0.40
Spleen
Absolute weight	Gram (10^−3)	0.80±0.14	0.75±0.11	0.78±0.15	0.79±0.10	0.50±0.09	0.52±0.08	0.56±0.10	0.55±0.04
Ratio per body weight		1.77±0.28	1.67±0.21	1.77±0.34	1.81±0.32	1.87±0.35	1.93±0.25	2.09±0.30	2.07±0.23
Ratio per brain		0.37±0.07	0.34±0.05	0.36±0.06	0.38±0.06	0.24±.04	0.26±0.04	0.27±0.05	0.27±0.02
Testes
Absolute weight	Gram (10^−3)	1.60±0.23	1.64±0.13	1.52±0.38	1.71±0.13	0.04±0.02	0.04±0.01	0.04±0.01	0.05±0.02
Ratio per body weight		3.57±0.66	3.65±0.34	3.44±0.82	3.92±0.58	0.14±0.07	0.14±0.06	0.16±0.05	0.19±0.06
Ratio per brain		0.74±0.14	0.75±0.08	0.70±0.16	0.82±0.06	0.02±0.01	0.02±0.01	0.02±0.00	0.02±0.01
Thymus
Absolute weight	Gram (10^−3)	0.28±0.04	0.29±0.05	0.26±0.06	0.23±0.06	0.21±0.03	0.20±0.06	0.21±0.08	0.19±0.04
Ratio per body weight		0.63±0.11	0.64±0.10	0.58±0.13	0.51±0.10	0.77±0.09	0.71±0.19	0.78±0.28	0.72±0.13
Ratio per brain		0.13±0.02	0.13±0.02	0.12±0.03	0.11±0.03	0.10±0.01	0.10±0.03	0.10±0.04	0.09±0.02
Lung
Absolute weight	Gram (10^−3)	1.67±0.25	1.68±0.18	1.88±0.35	1.68±0.13	1.31±0.17	1.39±0.30	1.28±0.12	1.30±0.11
Ratio per body weight		3.71±0.54	3.74±0.35	4.29±0.90	3.82±0.32	4.84±0.44	5.19±1.37	4.86±0.58	4.88±0.35
Ratio per brain		0.77±0.12	0.77±0.10	0.87±0.16	0.81±0.09	0.64±0.08	0.69±0.16	0.63±0.06	0.63±0.06

After the 13-week treatment, the wet organ weights and related organ weight to body weight (or brain weight) remained unaffected as compared to control group. Data were expressed as Mean ± S.D. (𝑁=10). Statistical analysis: ^∗𝑃<0.05,^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.001 indicate a statistical difference with the control group by one-way ANOVA.