Induction of Apoptosis by Luteolin Involving Akt Inactivation in Human 786-O Renal Cell Carcinoma Cells
Figure 1
Effects of luteolin on cell viability. 786-O cells were treated with various concentrations of luteolin. Representative images of phase contrast were obtained 24 h after treatment. μm (a). 786-O cells were treated with various concentrations of luteolin over time. Cell viability was determined by MTS reduction assay (b). 786-O cells were treated with various concentrations of luteolin for 24 h. The cells were harvested and processed by flow-cytometric analysis. The percentage of subG1 population is shown (c). Protein extracts were isolated and subjected to Western blot analysis with indicated antibodies. One of four independent experiments is shown (d). Protein extracts were isolated and subjected to fluorogenic caspase-3 assay. The intensity of fluorescent signals was expressed as arbitrary unit (e). ** versus medium control, .
