Induction of Apoptosis by Luteolin Involving Akt Inactivation in Human 786-O Renal Cell Carcinoma Cells
Figure 4
Role of HSP90. 786-O cells were treated with various concentrations of luteolin for 24 h. Protein extracts were isolated and subjected to Western blot analysis with indicated antibodies. One of four independent experiments is shown. An additional band of HSP90 was indicated by arrow (a). 786-O cells were treated with various concentrations of 17-AAG for 24 h. Cell viability was determined by MTS reduction assay (b). Protein extracts were isolated and subjected to fluorogenic caspase-3 assay. The intensity of fluorescent signals was expressed as arbitrary unit. ** versus medium control, (c). Protein extracts were isolated and subjected to Western blot analysis with indicated antibodies. One of four independent experiments is shown (d). Proteins obtained from medium- and luteolin (40 μM)-treated cells were immunoprecipitated by anti-HSP90 antibody, and the immunoprecipitates were subjected to Western blot analysis with indicated antibodies. One of four independent experiments is shown (e).
