Induction of Apoptosis by Luteolin Involving Akt Inactivation in Human 786-O Renal Cell Carcinoma Cells
Figure 5
Effects of agents on HSP90 cleavage. 786-O cells were treated with luteolin (0 and 40 μM) for 1 h, 3 h, and 24 h. Protein extracts were isolated and subjected to Western blot analysis with indicated antibodies. One of four independent experiments is shown (a). 786-O cells were treated with various concentrations of luteolin for 1 h and 24 h. Protein extracts were isolated and subjected to enzymatic assay for measurement of PP2A. ** versus medium control, (b). 786-O cells were pretreated with medium, SB203580, SP600125, LY294002, or Z-VAD-FMK for 1 h and then were treated with luteolin for additional 24 h. Protein extracts were isolated and subjected to Western blot analysis with indicated antibodies. An additional band of HSP90 was indicated by arrow. One of four independent experiments is shown (c).
