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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 120261, 11 pages
http://dx.doi.org/10.1155/2013/120261
Research Article

Baicalin’s Therapeutic Time Window of Neuroprotection during Transient Focal Cerebral Ischemia and Its Antioxidative Effects In Vitro and In Vivo

1College of Basic Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
2Xiyuan Hospital of China Academy of Chinese Medical Sciences, Beijing 100091, China
3Dongfang Hospital, The Second Clinical Medical College of Beijing University of Chinese Medicine, Beijing 100078, China
4The Central Hospital of Handan, Handan, Hebei 056001, China

Received 26 January 2013; Accepted 11 May 2013

Academic Editor: Hao Xu

Copyright © 2013 Fafeng Cheng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We investigated the effects of baicalin on an ischemia-reperfusion-induced brain injury model in rats and its antioxidative activities in vitro and in vivo. An ischemia-reperfusion injury of the brain via a middle cerebral artery occlusion (MCAO) was induced in rats. Baicalin was injected at different time points (0, 2, 4, and 6 h) after the MCAO was induced. Baicalin can improve neurological function and significantly decrease brain infarction within a time window of 4 h. Moreover, baicalin was able to reduce cell apoptosis and had the strong antioxidative effect of reducing reactive oxygen species production and malondialdehyde generation. In contrast, baicalin interfered with superoxide dismutase and nicotinamide adenine dinucleotide 2′-phosphate oxidase activities. Moreover, baicalin also exhibited strong neuroprotective effects against H2O2-mediated injury and improved the SOD activity of neurons. Furthermore, baicalin demonstrated good scavenging of hydroxyl radicals, superoxide anions, and DPPH radicals and exerted an additional effect of inhibiting xanthine oxidase. Baicalin showed beneficial effects against MCAO-induced injury within a 4 h time window, and its antioxidative effects both in vitro and in vivo may partly elucidate its mechanism of action.