Molecular Mechanisms Underlying the In Vitro Anti-Inflammatory Effects of a Flavonoid-Rich Ethanol Extract from Chinese Propolis (Poplar Type)
Figure 3
Effects of EECP on iNOS, IL-1β and IL-6 mRNA expression in LPS-stimulated RAW 264.7 cells. Dose-dependent (a) and time-course (b) inhibitory effect of EECP were measured on LPS-induced iNOS IL-1β and IL-6 mRNA expression in RAW 264.7 cells were measured using qRT-PCR, as described in Section 2. (a) Cells were pretreated with EECP (15 μg/mL) or not for 1 h, then stimulated with LPS (1 μg/mL) at various time points. (b) Dose-effect relationship was measured after 6 h stimulation with LPS (1 μg/mL). Results are expressed as a target gene expressions ratio comparing with the LPS-treated group. The data are the means ± SDs for three independent experiments. Individual groups were compared using student’s -test (*, **, *** compared with the LPS group; # compared with untreated group).
