Figure 1: SFN prevents GM-induced death in LLC-PK1 cells. (a–d) Representative images (10x) of LLC-PK1 cells obtained by phase contrast microscopy and (e, f) cell viability (MTT reduction) in the groups under study. A decrease in the number of cells exposed to (c) 8 mM GM for 72 h related to (a) cells without treatment can be observed. This decrease was prevented in (d) cells pretreated with 5 μM SFN for 24 h and coincubated with 5 μM SFN and 8 mM GM for 72 h. (e) Effect of SFN on viability of LLC-PK1 cells. Cells were incubated with 1–10 μM SFN for 96 h. (f) SFN prevents GM-induced cell death. Cells were incubated with 1–10 μM SFN for 24 h and then they were coincubated with 1–10 μM SFN and 8 mM GM for 72 h. Control cells were incubated (e) without SFN or (f) without SFN and GM. Data are . , versus control cells; , versus GM-treated cells.