Figure 2: SFN attenuates GM-induced viability drop in LLC-PK1 cells. Cells were incubated with 5 μM SFN for 24 h (SFN and SFN + GM) and then they were co-incubated with 5 μM SFN (SFN and SFN + GM) and 8 mM GM for 72 h (GM and SFN + GM). Control cells (CT) were incubated without SFN and GM. Viability was determined by the trypan blue exclusion assay. Data are mean ± SEM. versus CT; versus GM.