Sulforaphane Attenuates Gentamicin-Induced Nephrotoxicity: Role of Mitochondrial Protection

Table 1

Counting of daily detached, trypan blue stained and viable LLC-PK1 cells during the GM exposure and SFN treatment.

	CT	SFN	GM	SFN + GM
DC/well 24 h	1,483 ± 367	483 ± 60	5,487 ± 247*	1,113 ± 567^#
DC/well 48 h	1,633 ± 109	1,117 ± 469	8,033 ± 324*	4,250 ± 375^#
DC/well 72 h	2,867 ± 192	2,883 ± 259	8,017 ± 760^ε	4,733 ± 838^Δ
TBS cells/well (attached after GM exposure)	16,517 ± 3,219	23,733 ± 8,020	31,800 ± 3,318	25,433 ± 829
Total death cells/well	22,500 ± 3,399	28217 ± 8,366	53,337 ± 3,714^ε	35,550 ± 1,422
Viable cells/well	106,450 ± 4,976	94,317 ± 5,874	26,883 ± 4,681*	60,400 ± 5,765^Δ

CT: cells without treatment. SFN: cells incubated in 5 µM SFN. GM: cells in 8 mM GM. SFN + GM: cells coincubated in 5 µM SFN and 8 mM GM. DC: detached cells. TBS: trypan blue stained. Total death cells = DC 24 h + DC 48 h + DC 72 h + TBS cells. Cells attached after GM exposure include TBS cells + viable cells. Data are mean ± SEM, 𝑛=3. ^∗𝑃<0.001 versus CT; ^#𝑃<0.001 versus GM; ^𝜀𝑃<0.01 versus CT; ^Δ𝑃<0.05 versus GM.