Effect of Nelumbo nucifera Petal Extracts on Lipase, Adipogenesis, Adipolysis, and Central Receptors of Obesity

Table 2

Effect of petal extracts of N.  nucifera on central receptors.

Name of the test item	Solvent	Concentration	RLU (Mean ± SD)
			5HT2C	MC4R	MCHR1	CNR2
Negative	—	NA	35947.0±410.12	2322.3±111.11	NA	NA
DMSO	—	0.1%	36188.5±3324.11	2405.3±246.41	NA	NA
N.  nucifera methanol extract	DMSO	10 𝜇g/mL	146121.5±3987.37^∗	2276.5±289.21 (NS)	321.5±38.89 (NS)	106480.0±2791.66^∗
N.  nucifera successive water extract	DMEM	10 𝜇g/mL	150760.5±15262.89^∗	2071.5±282.14 (NS)	320.0±46.67 (NS)	122709.0±6588.82 (NS)
Agonist AL-34662; (reference standard)	DMSO	50 nm*	136099.9±2258.78^∗	NA	NA	NA
Agonist melanotan II acetate salt; (reference standard)	DMSO	950 nm*	NA	11798.5±27.58^∗	NA	NA
Agonist CP 55,940	DMSO	4.5 nm^1	NA	NA	NA	144375.5±7012.38
Agonist MCH	DMSO	62.5 nm^1	NA	NA	545.0±1.41	NA
Antagonist AM630; (reference standard)	DMSO	173.2 nm*	NA	NA	NA	4025.4±994.31^∗
Antagonist GW-803430; (reference standard)	DMSO	18.1 nm*	NA	NA	1294.5±142.13^∗	NA

RLU: relative luminescence units; NA: not applicable; *indicates a concentration at which maximum response of reference controls was observed; ¹indicates EC₈₀ concentration of agonist against which N.  nucifera petal extracts/reference controls were evaluated for their antagonistic potential.
𝑃<0.05.
NS: not significant.