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Evidence-Based Complementary and Alternative Medicine
Volume 2013, Article ID 147362, 14 pages
Research Article

A Systematic, Integrated Study on the Neuroprotective Effects of Hydroxysafflor Yellow A Revealed by NMR-Based Metabonomics and the NF- B Pathway

1State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China
2School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China

Received 5 February 2013; Accepted 2 March 2013

Academic Editor: Aiping Lu

Copyright © 2013 Yuanyan Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Table 1S: Sequence of primers.

Table 2S: Neurological deficit scores of different administrations of HSYA were observed in vehicle-treated MCAO rats.

Table 3S: 1H NMR Chemical shifts of main metabolites contributing to the classification of ischemia in different brain tissues.

Figure 1S: Loading plots from the OPLS-DDA of the NMR spectra of (a)–(b) cerebellum, (c)–(d) cortex and (e)–(f) hippocampus. Note the most similar metabolic changes that occurred within these tissues following ischemia. These loading plots corresponded to the scores plots in Fig. 4 (a), ((b), (d), (e), (g) and (h), respectively. Positive peaks corresponded to metabolites that were at higher concentration in ischemia animals and were assigned as described in the legend to Figure 3.

Figure 2S: Effect of HSYA and PDTC on NF-κB binding activity after pMCAO. Data represent x ± s . n = 6 each group; results represent at least three independent experiments. ## p<0.01 compared with sham group. * p 0.05, ** p<0.01 compared with model group.

  1. Supplementary Materials